Sažetak
Uvod/Cilj. Poznato je da se neke biljne vrste roda Convolvulus (C) L. često koriste u turskoj narodnoj medicini kao snažni agensi u lečenju zubobolje i kod bolova u zglobovima. Zbog ograničenih informacija o biološkim aktivnostima C. aucheri, vrste koja se gotovo isključivo javlja u Turskoj, cilj rada bio je ispitivanje antioksidativnog potencijala, kao i citotoksičnih svojstava tri različita ekstrakta, uz karakterizaciju njihovog fenolnog profila. Metode. Antioksidativna aktivnost ekstrakta određena je korišćenjem ABTS, NO, FRAP, fosfomolibdenskog i testa heliranja metala. Pored toga, u ekstraktima su identifikavana i određena sledeća bioaktivna jedinjenja: ukupni fenolni, flavonoidi i saponini. Citotoksičnost je procenjena primenom CellTiter-Glo testa na kancerskim HeLa i H1299 ćelijskim linijama. Rezultati. Metanolni ekstrakt C. aucheri ispoljio je najveću antioksidativnu aktivnost, a u njemu je utvrđen i najveći sadržaj fenola, flavonoida i saponina. Tečnom hromatografijom pod visokim pritiskom (HPLC) utvrđeno je da su glavna jedinjenja u ovom ekstraktu hlorogenska kiselina, (+)-katehin, ruzmarinska kiselina i rutin. Otkriveno je da metanolni ekstrakt dobijen iz nadzemnih delova C. aucheri smanjuje vitalnost HeLa ćelija, a određena IC50 vrednost bila je 14,22 µg/mL. Zaključak. Naši rezultati su pokazali da bi C. aucheri mogao biti dobar novi i alternativni izvor prirodnih antioksidanasa i jedinjenja sa citotoksičnim efektom.
Ključne reči
convolvulus
hromatografija, tečna, pod visokim pritiskom
fitoterapija
turska
Reference
Rodrigues T, Reker D, Schneider P, Schneider G. Counting on natural products for drug design. Nat Chem 2016; 8(6): 531–41.
Bahadori MB, Kirkan B, Sarikurkcu C. Phenolic ingredients and therapeutic potential of Stachys cretica subsp. smyrnaea for the management of oxidative stress, Alzheimer’s disease, hypergly-cemia, and melasma. Ind Crops Prod 2019; 127(1): 82–7.
Al-Snafi AE. Medicinal plants with antioxidant and free radi-cal scavenging effects (part 2): a plant-based review. IOSR J Pharmacy 2016; 6(7): 62–82.
Aykurt C. Taxonomical studies on genus Convolvulus L. (Convolvulaceae) in Turkey [thesis]. Antalya, Turkey: Univer-sity of Akdeniz, Department of Biology; 2010.
Altundag E, Ozturk M. Ethnomedicinal studies on the plant re-sources of east Anatolia, Turkey. Proced Soc Behav Sci 2011; 19: 756–77.
Aykurt C, Sümbül H. Taxonomic revision of the genus Con-volvulus L. (Convolvulaceae) in Turkey. Biodivers Conserv 2014; 7(2): 10–37.
Rachitha P, Krupashree K, Jayashree GV, Kandikattu HK, Amruta N, Gopalan N, et al. Chemical composition, antioxidant po-tential, macromolecule damage and neuroprotective activity of Convolvulus pluricaulis. J Tradit Complement Med 2018; 8(4): 483–96.
Atta AH, El-Sooud KA. Antinociceptive effect of some Egyp-tian medicinal plant extracts. J Ethnopharmacol 2004; 95(2‒3): 235–8.
Sadeghi-Aliabadi H, Ghasemi N, Kohi M. Cytotoxic effect of Convolvulus arvensis extracts on human cancerous cell line. Res Pharm Sci 2008; 3(1): 31–4.
Elzaawely AA, Tawata S. Antioxidant Activity of Phenolic Rich Fraction Obtained from Convolvulus arvensis L. Leaves Grown in Egypt. Asian J Crop Sci 2012; 4(1): 32–40.
Benmerache A, Berrehal D, Kabouche A, Semra Z, Thomas O, Tou-zani R, et al. Antioxidant, antibacterial activities and flavo-noids of Convolvulus fatmensis G. Kunze. Der Pharm Lett 2013; 5(1): 371–5.
Ozay C. In Vitro Antioxidant Property of Convolvulus aucheri and Its NO Inhibitory Potential in LPS-activated NSCLC Cells. J Inst Sci Tech 2019; 9(3): 1282‒8.
Slinkard K, Singleton VL. Total phenol analyses: automation and comparison with manual methods. Am J Enol Vitic 1977; 28(1): 49–55.
Moreno MI, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 2000; 71(1‒2): 109–14.
Hiai S, Oura H, Nakajima T. Color reaction of some sapogen-ins and saponins with vanillin and sulfuric acid. Planta Med 1976; 29(2): 116–22.
Caponio F, Alloggio V, Gomes T. Phenolic compounds of virgin olive oil: influence of paste preparation techniques. Food Chem 1999; 64(2): 203–09.
Zengin G, Nithiyanantham S, Locatelli M, Ceylan R, Uysal S, Ak-tumsek A, et al. Screening of in vitro antioxidant and enzyme inhibitory activities of different extracts from two uninvesti-gated wild plants: Centranthus longiflorus subsp. longiflorus and Cerinthe minor subsp. auriculata. Eur J Integr Med 2015; 8(3): 286–92.
Berk S, Tepe B, Arslan S, Sarikurkcu C. Screening of the anti-oxidant, antimicrobial and DNA damage protection potentials of the aqueous extract of Asplenium ceterach DC. Afr J Bio-technol 2011; 10(44): 8902–8.
Balakrishnan N, Panda AB, Raj NR, Shrivastava A, Prathani RB. The evaluation of nitric oxide scavenging activity of Acalypha indica Linn Root. Asian J Res Chem 2009; 2(2): 148–50.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999; 26(9-10): 1231–37.
Ebrahimzadeh MA, Tavassoli A. Antioxidant properties of Hel-ichrysum pseudoplicatum Nab. Pharm Biomed Res 2015; 1(1): 37–43.
Todd FG, Stermitz FR, Schultheis P, Knight AP, Traub-Dargatz J. Tropane alkaloids and toxicity of Convolvulus arvensis. Phy-tochemistry 1995; 39(2): 301–3.
Menemen Y, Williams CA, Jury SL. Flavonoid patterns in Con-volvulus L. (Convolvulaceae) species from Morocco. Pak J Botany 2002; 34(3): 291–5.
Turker AU, Yıldırım AB. Clonal propagation, antioxidant ac-tivity and phenolic profiles of Convolvulus galaticus Rostan ex Choisy. Rom Biotech Lett 2018; 23(3): 13625–36.
El Raheim Mohammed Donia A, Alqasoumi SI, Awaad AS, Cracker L. Antioxidant activity of Convolvulus hystrix Vahl and its chemical constituents. Pak J Pharm Sci 2011; 24(2): 143‒7.
Hegab MM, Ghareib HR. Methanol Extract Potential of Field Bindweed (Convolvulus arvensis L) for Wheat Growth En-hancement. Int J Botany 2010; 6(3): 334–42.
Bender O, Atalay A. Evaluation of anti-proliferative and cyto-toxic effects of chlorogenic acid on breast cancer cell lines by real-time, label-free and high-throughput screening. Marmara Pharm J 2018; 22(2): 173–9.
Ma Y, Ding S, Fei Y, Liu G, Jang H, Fang J. Antimicrobial ac-tivity of anthocyanins and catechins against foodborne patho-gens Escherichia coli and Salmonella. Food Control 2019; 106: 106712.
Yang J, Guo J, Yuan J. In vitro antioxidant properties of rutin. LWT Food Sci Technol 2008; 41(6): 1060‒6.
Oksana S, Marian B, Mahendra R, Bo SH. Plant phenolic com-pounds for food, pharmaceutical, and cosmetics production. J Med Plant Res 2012; 6(13): 2526–39.
Al-Dabbas MM. Antioxidant activity of different extracts from the aerial part of Moringa peregrina (Forssk.) Fiori, from Jordan. Pak J Pharm Sci 2017; 30(6): 2151–7.
Al-Rifai A, Aqel A, Al-Warhi T, Wabaidur SM, Al-Othman ZA, Badjah-Hadj-Ahmed AY. Antibacterial, Antioxidant Activity of Ethanolic Plant Extracts of Some Convolvulus Species and Their DART-ToF-MS Profiling. Evid Based Complement Al-ternat Med 2017; 2017: 5694305.
Thakral J, Borar S, Kalia AN. Antioxidant potential fractiona-tion from methanol extract of aerial parts of Convolvulus arvensis Linn. (Convolvulaceae). Int J Pharm Sci Drug Res 2010; 2(3): 219–23.
Awaad AS, Al-Jaber N. Antioxidant natural plant. RPMP Ethnomed Source Mechanism 2010; 27: 1–35.
Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, et al. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Can-cer 2015; 136(5): E359‒86.
Shukla D, Rawal R, Jain N. A brief review on plant-derived natural compounds as anti-cancer agents. Int J Herb Med 2018; 6(5): 28‒36.
Saleem M, Qadir MI, Ahmad B, Saleem U, Naseer F, Schini Kerth V, et al. Cytotoxic effect of ethanol extract of Convolvulus arvensis L (Convolvulaceae) on lymphoblastic leukemia Jurkat cells. Trop J Pharm Res 2014; 13(5): 705–9.
AL-Asady AAB, Suker DK, Hassan KK. Cytotoxic and cytoge-netic effects of Convolvulus arvensis extracts on rhabdomyosar-coma (RD) tumor cell line in vitro. J Med Plants Res 2014; 8(15): 588–98.
Kaur M, Kalia AN. Anticancer potential of the Convolvulus arvensis. Int J Pharm Res All Sci 2012; 1(3): 101–2.
Meng X, Riordan N, Casciari J, Zhu Y, Zhong J, González M, et al. Effects of a high molecular mass Convolvulus arvensis ex-tract on tumor growth and angiogenesis. P R Health Sci J 2002; 21(4): 323–8.
Calvino N. Anti-angiogenesis properties of a common weed, Convolvulus arvensis. J Chiropr Med 2002; 1(3): 116.
Al-Bowait ME, Albokhadaim IF, Homeida AM. Immunostimu-lant Effect of Bindweed (Convolvulus arvensis) Extract in Rabbit. Res J Pharmacol 2010; 4(2): 51–4.
Man S, Gao W, Zhang Y, Huang L, Liu C. Chemical study and medical application of saponins as anticancer agents. Fitotera-pia 2010; 81(7): 703–14.
Joseph A, Dhanraj K, Ramalıngam S, Saravanan R. Treating cer-vical cancer with herbs: a review. Asian J Pharm Clin Res 2018; 11(7): 4–10.
Xu W, Liu J, Li C, Wu HZ, Liu YW. Kaempferol-7-O-D-glucoside (KG) isolated from Smilax china L. rhizome induces G 2/M phase arrest and apoptosis on HeLa cells in a p53-independent manner. Cancer Lett 2008; 264(2): 229‒40.