Journal of Medical Biochemistry
type: channel
publisher: Society of Medical Biochemists of Serbia
language: sr-Latn
publication name: Journal of Medical Biochemistry
issn: 1452-8258
copyright: The published articles will be distributed under the Creative Commons Attribution 4.0 International License (CC BY). It is allowed to copy and redistribute the material in any medium or format, and remix, transform, and build upon it for any purpose, even commercially, as long as appropriate credit is given to the original author(s), a link to the license is provided and it is indicated if changes were made. Users are required to provide full bibliographic description of the original publication (authors, article title, journal title, volume, issue, pages), as well as its DOI code. In electronic publishing, users are also required to link the content with both the original article published in Journal of Medical Biochemistry and the licence used. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
items: Molecular and Biochemical Characterization of Peripheral Blood MMP-2 Upregulation and TIMP-2 Downregulation in In-Stent Restenosis
items: Prognostička vrednost biomarkera vezanih za feroptozu ACSL4 i GPKS4 kod kritično bolesnih pacijenata: sistematski pregled i meta-analiza
items: Анализа корелације нивоа CD354 и ANGPT2 у серуму са прогнозом сепсе
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items: Улога серумских антитела BAFF, CFB, MCP-1 и анти-ПЛА2R у процени ефикасности код пацијената са мембранозном нефропатијом
items: Серумски кератин 1 и ТМАП у прогнози срчане функције акутне срчане инсуфицијенције (АХФ)
items: Анализа укорељености серума SERPINA3, CLEC2 и hs-CRP/ALB са главним нежељеним кардиоваскуларним догађајима (MACE) након перкутане коронарне интервенције (PCI) код STEMI
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items: Redoks disbalans u neutrofilima u akutnom koronarnom sindromu
items: Анализа корелације између нивоa CCR5 и LXA4 у серуму и ремоделирања дијафрагмалног пута kod pacijenata sa астмом
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items: The Novel Serum Biomarkers for Real-Time Treatment Monitoring and Prognostication in Hepatocellular Carcinoma Patients
items: Digitalni Biomarkeri i Medicinski Algoritmi u Srpskom Zdravstvenom Sistemu: Komparativna analiza znanja, stavova, prakse i ekonomske valuacije među ključnim zdravstvenim profesionalcima
items: Diagnostic Value of Integrated Serum Protein Carbonyl, 8-Hydroxy-2'-Deoxyguanosine, and PGC-1α Profiling for Myocardial Energy Metabolism Disorder in Ischemic Cardiomyopathy
items: Klinički i biohemijski prognostički značaj cirkulirajućih biomarkera povezanih sa tumorom kod pacijenata sa karcinomom pluća sa komorbidnom hroničnom opstruktivnom plućnom bolešću
items: the Effect of Treatment on Serum C3, C4, IL-10, IL1-β, IL-6, TNF-α in Patients with Systemic Lupus Erythematosus
items: Анализа корелације нивоа сира СИР2Л4, Ц1КТНФ5 и ЦБП-35 у серуму са прогнозом пацијената са дијабетичком ретинопатијом
items: Дијагностичка вредност серума 25-хидроксивитамин Д, МИС, инсулин-сличан фактор раста 1 за синдром полицистичних јајника (ПЦОС)
items: Ефекти нивоа тромбоспондина-1 (THBS1) и тол-лика рецептора 4 (TLR-4) у серуму и синовијалној течности код пацијената са остеоартритисом колена
items: Uticaj autoverifikacije na vreme izdavanja rezultata za kliničko-hemijske i imunoesejske testove
items: PRIMENJIVOST FORMULA ZA IZRAČUAVANJE LDL-HOLESTEROLA U HIPERTRIGLICERIDEMIJI: UVIDI IZ VOJVODINE
items: Анализа корелације нивоа експресије сировинског молекула за адхезију ендотелних ћелија-1 и протеина сиртуин 1 код акутног респираторног дистрес синдрома
items: Clinical significance of miR-3178 in non-small cell lung cancer patients
items: Serum OKS40 Ligand kao biohemijski regulator Th2-dominantne imune aktivacije u astmi: integrisana analiza citokinske mreže
items: Procena nivoa alfa klotoa u plazmi kod gojaznih slučajeva
items: Profili biohemijskih i inflamatornih biomarkera u serumu u teškoj preeklampsiji i njihova klinička prediktivna vrednost
items: Анализа корелације серумског ФГЛП1, УАЦР и ризика од срчане инсуфицијенције код пацијената са дијабетесом типа 2
items: Анализа корелације серума CCL3, TRACP-5b и склеростина са прогнозом пацијената са мултиплим миеломом
items: Predviđanje promene morfologije n. vagus-a na osnovu biohemijski markera upale u COVID 19 infekciji
items: Dijagnostika hronične bubrežne bolesti u Srbiji: aktuelna laboratorijska praksa i prioriteti harmonizacije

Molecular and Biochemical Characterization of Peripheral Blood MMP-2 Upregulation and TIMP-2 Downregulation in In-Stent Restenosis
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description: Background: The matrix metalloproteinase-2/tissue inhibitor of metalloproteinase-2 (MMP-2/TIMP-2) axis is an important regulator of extracellular matrix turnover and vascular remodeling. Most previous studies have focused on either circulating protein levels or mRNA expression alone. Because PBMC mRNA expression and plasma protein concentration reflect different regulatory levels, paired cross-level analysis may provide a more complete characterization of peripheral blood MMP-2/TIMP-2 alterations in ISR. This study investigated the molecular and biochemical dysregulation of the peripheral blood MMP-2/TIMP-2 axis in patients with ISR. Methods: A total of 117 patients who underwent follow-up evaluation 6–12 months after coronary stent implantation were included, comprising 42 patients with ISR and 75 without ISR. Plasma MMP-2 and TIMP-2 concentrations were measured by ELISA, and MMP-2 and TIMP-2 mRNA expression levels in peripheral blood mononuclear cells were quantified by qPCR. Between-group differences, transcription–protein coupling, and associations with routine biochemical parameters were analyzed. Results: Patients with ISR showed increased MMP-2 expression and decreased TIMP-2 expression at both the mRNA and protein levels compared with patients without ISR. The altered MMP-2/TIMP-2 balance was consistent across transcriptional and protein measurements. TIMP-2 showed an inverse association with LDL-C at both levels, whereas the relationships between MMP-2-related measurements and hs-CRP, Hcy, and Lp(a) suggested heterogeneous biochemical regulation. Standardized qPCR- and ELISA-derived measurements showed consistent cross-level patterns, supporting the presence of parallel molecular and protein-level alterations of this axis. Conclusion: ISR is associated with a consistent peripheral blood MMP-2/TIMP-2 imbalance, involving increased MMP-2 expression and decreased TIMP-2 expression at both the mRNA and circulating protein levels. These findings provide pathobio…
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creator: Xinghu Zhou
creator: Hongbin Ma
creator: Xuan Qiao
creator: Bai Bai
rights: Sva prava zadržana (c) 2026 Xinghu Zhou, Hongbin Ma, Xuan Qiao, Ming Bai
date: 2026-07-09
publication date: 2026-07-09
doi: 10.5937/jomb0-67085

Prognostička vrednost biomarkera vezanih za feroptozu ACSL4 i GPKS4 kod kritično bolesnih pacijenata: sistematski pregled i meta-analiza
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description: Background: Ferroptosis is a regulated form of cell death characterized by iron-dependent lipid peroxidation and has been increasingly implicated in the pathogenesis of severe infections and critical illness. Acyl-CoA synthetase long-chain family member 4 (ACSL4) and glutathione peroxidase 4 (GPX4) are key regulators of ferroptosis and may serve as clinically useful biomarkers for prognostic assessment. This systematic review and meta-analysis aimed to evaluate the prognostic significance of serum ACSL4 and GPX4 levels in critically ill patients with severe infections. Methods: A systematic search of PubMed, Embase, Web of Science, Cochrane Library, China National Knowledge Infrastructure (CNKI), Wanfang Data, and China Biology Medicine Disc (CBM) was conducted from database inception to March 2026. Observational studies evaluating the association between serum ACSL4 and/or GPX4 levels and clinical outcomes in critically ill patients were included. Study quality was assessed using the Newcastle–Ottawa Scale (NOS). Meta-analysis was performed using RevMan 5.4 and Stata 16.0. Odds ratios (ORs) and 95% confidence intervals (CIs) were pooled using random-effects models. Diagnostic performance was evaluated using receiver operating characteristic (ROC) data where available. Heterogeneity, sensitivity analyses, and publication bias assessments were conducted according to standard methodological approaches. Results: A total of 42 records were identified, and two high-quality prospective cohort studies involving 437 critically ill patients were included. NOS scores ranged from 8 to 9 points. Meta-analysis demonstrated that ferroptosis-related biomarkers were significantly associated with short-term mortality. Reduced serum GPX4 (OR=1.076, 95% CI: 1.036–1.119), reduced GSH/GSSG ratio (OR=1.258, 95% CI: 1.161–1.364), and elevated serum ACSL4 (OR=4.00, 95% CI: 2.00–8.00) were independently associated with adverse outcomes. The pooled OR was 1.76 (95% CI: 1.03–3.01), indicating…
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creator: Yue Gao
creator: Chengxiang Wang
rights: Sva prava zadržana (c) 2026 Yue Gao, Chengxiang Wang
date: 2026-07-07
publication date: 2026-07-07
doi: 10.5937/jomb0-68002

Анализа корелације нивоа CD354 и ANGPT2 у серуму са прогнозом сепсе
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description: [Objective] To explore the changes of procalcitonin (PCT), Angiopoietin-2 (ANGPT2), and serum Cluster of Differentiation 354 (CD354) concentrations in pediatric sepsis patients. [Methods] 190 children with sepsis who were hospitalized to our hospital between January 2024 and June 2025 had their clinical data analyzed retrospectively. Based on the severity of their conditions, the patients were divided into a severe group (n = 84) and a nonsevere group (n = 106). A survival group (n = 134) and a death group (n = 56) were created based on the prognosis of the two groups of children within 28 days after admission. Serum levels of CD354, ANGPT2, and PCT in the severe and nonsevere groups were compared, and the predictive efficacy of these indicators alone or in combination for assessing the severity of sepsis in children was determined using receiver operating characteristic (ROC) curves. The differences in serum CD354, ANGPT2 and PCT levels between the survival group and the death group were compared, and the predictive efficacy of these indicators alone or in combination for evaluating the prognosis of sepsis in children was determined using receiver operating characteristic (ROC) curves. [Results] The serum CD354, ANGPT2 and PCT levels were higher in the severe group than in the nonsevere group (P < 0.05). The sensitivity and specificity of serum CD354 for evaluating the severity of sepsis in children were 92.48% and 66.60%, respectively. The sensitivity and specificity of serum ANGPT2 for evaluating the severity of sepsis in children were 94.37% and 88.13%, respectively. The sensitivity and specificity of serum PCT for evaluating the severity of sepsis in children were 94.37% and 66.60%, respectively. The sensitivity and specificity of serum ANGPT2 for evaluating the severity of sepsis in children were 94.37% and 88.13%, respectively. The sensitivity and specificity of serum PCT for evaluating the prognosis of sepsis in children were 64.22% and 100.00%, respectively…
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creator: Mingying Luo
creator: Yun Hong
creator: Junhao Chen
creator: Cong Fu
rights: Sva prava zadržana (c) 2026 Mingying Luo, Yun Hong, Junhao Chen, Cong Fu
date: 2026-05-19
publication date: 2026-05-19
doi: 10.5937/jomb0-65632

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creator: Zhongqing Wang
creator: Bin Sun
creator: Wei Wang
creator: Tinglin Xiong
creator: Gang Wei
rights: Sva prava zadržana (c) 2026 Zhongqing Wang, Bin Sun, Wei Wang, Tinglin Xiong, Gang Wei
date: 2026-07-07
publication date: 2026-07-07
doi: 10.5937/jomb0-67129

Улога серумских антитела BAFF, CFB, MCP-1 и анти-ПЛА2R у процени ефикасности код пацијената са мембранозном нефропатијом
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description: Objective: Monocyte chemoattractant protein-1 (MCP-1), serum B-cell activating factor (BAFF), complement B factor (CFB), and anti-M-type phospholipase A2 receptor (PLA2R) antibodies are investigated in connection with membranous nephropathy (MN) and their roles in evaluating the efficacy of treatment. Methods: 108 patients who were hospitalized to our hospital between January 2023 and June 2024 were chosen to serve as research participants. Patients were divided into remission and nonremission groups according to their clinical efficacy. The levels of serum BAFF, CFB, MCP-1 and anti-PLA2R antibodies in patients with positive and negative anti-PLA2R and different pathological stages were compared, and the clinical data of the remission group and the nonremission group were compared. The association between serum levels of BAFF, CFB, and MCP-1 and anti-PLA2R antibody levels in patients who tested positive for anti-PLA2R antibody. The predictive efficacy of serum anti-PLA2R antibodies, BAFF, CFB, and MCP-1 for nonremission in MN patients after therapy was evaluated using receiver operating characteristic (ROC) curves. Results: There were 78 patients who were positive for anti-PLA2R antibodies and 30 patients who were negative for anti-PLA2R antibodies. The levels of serum BAFF, CFB, MCP-1 and anti-PLA2R antibodies in patients positive for anti-PLA2R antibodies were significantly greater than those in patients negative for anti-PLA2R antibodies (P<0.05). The comparison of the serum BAFF, CFB and MCP-1 levels in MN patients at different stages were as follows: stage I < stage II < stage III < stage IV. Serum BAFF, CFB, and MCP-1 in patients positive for anti-PLA2R antibodies were positively correlated with the level of anti-PLA2R antibodies (r=0.792, 0.823, 0.832, P<0.001). Spearman correlation analysis revealed that the levels of serum BAFF, CFB, and MCP-1 in MN patients were positively correlated with pathological stage (rs=0.758, 0.752, 0.717, P<0.001). The pathologic…
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creator: Kaiyuan Zheng
creator: Tongxin Qu
creator: Lili Han
creator: Yi Sun
rights: Sva prava zadržana (c) 2025 Kaiyuan Zheng, Tongxin Qu, Lili Han, Yi Sun
date: 2025-12-10
publication date: 2025-12-10
starting page: 1420
ending page: 1429
doi: 10.5937/jomb0-61102

Серумски кератин 1 и ТМАП у прогнози срчане функције акутне срчане инсуфицијенције (АХФ)
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description: [Objective] To explore the relationships between serum TMAP (Tumor metastasis-associated peptide) and KRT1(Keratin 1) levels and the cardiac function classification and prognosis of patients with acute heart failure (AHF). [Methods] From January 2022 to January 2025, 428 patients with acute heart failure (AHF) admitted to this hospital composed the AHF group. Among them, 162 patients had a NYHA cardiac function classification of grade II, 166 patients had a grade III, and 100 patients had a grade IV. The control group consisted of 100 more healthy volunteers who had physical examinations at the same hospital during the same time period. After the AHF patients received relevant treatments according to the guidelines and were discharged, a 6-month follow-up was conducted. Patients with a poor prognosis were those who were readmitted due to heart failure or cardiac death; patients with a positive prognosis were those who were not. Each study participant's serum levels of KRT1 and TMAP were determined using the enzyme-linked immunosorbent assay (ELISA). Patients with AHF were given baseline data. The relationship between the classification of cardiac function in AHF patients and serum TMAP and KRT1 levels was examined using Spearman correlation analysis. The factors determining a poor prognosis in individuals with AHF were examined using multivariate logistic regression analysis. The capacity of serum TMAP and KRT1 levels to predict a bad prognosis in AHF patients was examined using receiver operating characteristic (ROC) curves. Comparisons of the area under the curve (AUC) were conducted using the DeLong test. [Results] Serum TMAP levels were greater in the AHF group than in the control group (P < 0.05), and serum KRT1 levels were lower in the AHF group than in the control group (P < 0.05). The serum TMAP level increased with increasing cardiac function classification (F trend = 109.195, P trend < 0.001). Conversely, as heart function categorization increased, the ser…
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creator: Chengqun She
creator: Juan Wu
creator: Yaoguang Feng
creator: Chaozhong Long
creator: Zusong Tan
rights: Sva prava zadržana (c) 2026 Chengqun She, Juan Wu, Yaoguang Feng, Chaozhong Long, Zusong Tan
date: 2026-07-06
publication date: 2026-07-06
doi: 10.5937/jomb0-66860

Анализа укорељености серума SERPINA3, CLEC2 и hs-CRP/ALB са главним нежељеним кардиоваскуларним догађајима (MACE) након перкутане коронарне интервенције (PCI) код STEMI
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description: Objective To examine the main adverse cardiovascular events that occur in individuals who have had an acute ST-segment elevation myocardial infarction (STEMI) after receiving percutaneous coronary intervention (PCI), such as serum C-type lectin domain family member 2 (CLEC2), serine protease inhibitor family member A3 (SERPINA3), and high-sensitivity C-reactive protein/albumin (hs-CRP/ALB) levels. Methods The STEMI group included 132 patients who were observed for a year after being hospitalized to the hospital between January 2023 and September 2024. The patients were divided into two groups: the MACE group and the non-MACE group, based on whether MACE came after PCI. Additionally, The control group consisted of 68 healthy people who were examined physically at the hospital throughout the same time period. Using an enzyme-linked immunosorbent test, the levels of serum CLEC2, SERPINA3, hs-CRP, and ALB were measured in each research participant, and the hs-CRP to ALB ratio was computed. After PCI, multivariate logistic regression was utilized to examine the variables affecting MACEs in STEMI patients. Receiver operating characteristic (ROC) curves were used to analyze the predictive value of single and combined detection of serum CLEC2, SERPINA3, and hs-CRP/ALB for MACEs in STEMI patients after PCI. Results The incidence of MACE after PCI in 132 STEMI patients was 31.06% (41/132). The levels of serum CLEC2, SERPINA3 and hs-CRP/ALB in the STEMI group were significantly greater than those in the control group (P<0.05). Independent risk factors for MACEs following PCI in STEMI patients included age ≥62 years, Killip grade ≥ grade III, cardiac troponin I level ≥1.7 ng/mL, CLEC2 level ≥155 pg/mL, SERPINA3 level ≥350 ng/L, and hs-CRP/ALB≥0.50 (P<0.05), while left ventricular ejection fraction ≥50% was an independent protective factor. The area under the ROC curve (0.856) of the combined detection of serum CLEC2, SERPINA3, and hs-CRP/ALB for the prediction of MACEs in STEMI…
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creator: Yuncong Ma
creator: Suxia Fang
creator: Shasha Liu
creator: Zhencong Jiang
rights: Sva prava zadržana (c) 2025 Yuncong Ma, Suxia Fang, Shasha Liu, Zhencong Jiang
date: 2025-11-17
publication date: 2025-11-17
starting page: 1259
ending page: 1268
doi: 10.5937/jomb0-62259

Error
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description: Error
subject: Original paper
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creator: Error
rights: Sva prava zadržana (c) 2026 Jiahao Chen, Wenwu Liu, Xinci Zhang, Chenchen Zhu
date: 2026-05-16
publication date: 2026-05-16
doi: 10.5937/jomb0-66215

Redoks disbalans u neutrofilima u akutnom koronarnom sindromu
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description: Kratak sadržaj Uvod: Oksidativni stres ima ključnu ulogu u patogenezi akutnog koronarnog sindroma (AKS), a neutrofili doprinose oštećenju krvnih sudova preko produkcije reaktivnih kiseoničnih radikala i oksidanasa zavisnih od mijeloperoksidaze (MPO). Cilj istraživanja je bio da se ispitaju promene markera oksidativnog stresa i aktivnosti antioksidativnih enzima u neutrofilima pacijenata sa AKS u poređenju sa kontrolnim subjektima. Metod: Neutrofili su izolovani iz krvi 77 pacijenata sa AKS i 33 kontrolna subjekta. Oksidativni stres je procenjen merenjem konjugovanih diena, hidroperoksida i hloramina. Antioksidativni status je ocenjen određivanjem nivoa neproteinskih i ukupnih tiolnih grupa, kao i aktivnosti superoksid dismutaze (SOD), katalaze, glutation peroksidaze (GPx-1) i glutation reduktaze (GR). Takođe su određivane peroksidazna i hlorinišuća aktivnost MPO. Rezultati: U grupi pacijenata sa AKS zabeležene su značajno više koncentracije konjugovanih diena (p < 0,005), hloramina (p < 0,005), tiolnih grupa (p < 0,05), aktivnosti SOD (p = 0,05) i hlorinišuće aktivnosti MPO (p < 0,05). Nisu nađene značajne razlike u aktivnostima katalaze, GPx-1, GR i peroksidazne aktivnosti MPO. Značajne korelacije uočene su između markera lipidne peroksidacije i antioksidativnih parametara, naročito SOD i MPO hlorinišuće aktivnosti. Višestruka regresiona analiza identifikovala je SOD i hloramine kao nezavisne prediktore lipidne peroksidacije. Zaključak: Neutrofili pacijenata sa AKS pokazuju izražen redoks disbalans, karakterisan pojačanom lipidnom peroksidacijom, povećanim sadržajem tiola i povećanom hlorinišućom aktivnošću MPO. Ovi nalazi ukazuju na potencijal redoks parametara neutrofila kao biomarkera i terapijskih meta u AKS. Ključne reči: akutni koronarni sindrom , neutrofili, oksidativni stress
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creator: Ilija M Dragojević
creator: Dijana Mirić
creator: Bojana Kisić
creator: Dragana Puhalo Sladoje
creator: Ljiljana Popović
creator: Dragiša Rašić
rights: Sva prava zadržana (c) 2025 Ilija M Dragojevic, Dijana Miric, Bojana Kisic, Dragana Puhalo Sladoje, Ljiljana Popovic, Dragisa Rasic
date: 2025-09-01
publication date: 2025-09-01
starting page: 357
ending page: 364
doi: 10.5937/jomb0-59659

Анализа корелације између нивоa CCR5 и LXA4 у серуму и ремоделирања дијафрагмалног пута kod pacijenata sa астмом
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description: [Objective] To analyze the expression levels of serum chemokine receptor 5 (CCR5) and lipoxin A4 (LXA4) in patients with bronchial asthma and their correlations with immune dysfunction and airway remodeling. [Methods] According to the severity of the disease, 240 patients with bronchial asthma admitted to our hospital from January 2023 to May 2025 were divided into mild to moderate asthma groups (n=180) and severe asthma groups (n=60). As the control group, 150 more healthy people who were admitted for a physical examination during that time were chosen. The serum CCR5 and LXA4 levels and immune function indicators (CD3+, CD4+, CD8+, and CD4+/CD8+) of each group were detected, and the airway remodeling indicators [airway lumen area (LA), airway wall area (WA) and total airway area (TA)] of the right upper lobe tip segment were examined via CT. The correlations between the serum CCR5 and LXA4 levels and immune dysfunction as well as airway remodeling were analyzed via a bivariate Spearman correlation test, and a multivariate logistic model was established to analyze the independent risk factors influencing the serum CCR5 and LXA4 levels in patients with bronchial asthma. [Results] Compared to the mild to moderate group, the severe group had greater serum levels of LXA4 and CCR5. Serum CCR5 was higher in the severe group than in the mild to moderate group, and LXA4 was lower in the severe group than in the mild to moderate group. There were statistically significant differences (P<0.05). Serum CD8+ T cell levels were higher than in the control group, whereas CD3+, CD4+, and CD4+/CD8+ T cell levels were lower in the mild to moderate and severe groups. Furthermore, the severe group had lower levels of CD3+, CD4+, and CD4+/CD8+ T cells than the mild to moderate group, whereas the mild to moderate group had greater levels of CD8+ T cells. The mild to moderate group of patients and the severe group of patients had lower levels of LA, WA, and TA in the right superior lobe a…
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creator: Haiyue Huang
creator: Ruirui Luo
creator: Xuan Yang
creator: Zhigang Wu
creator: Zhongxi Ding
rights: Sva prava zadržana (c) 2026 Haiyue Huang, Ruirui Luo, Xuan Yang, Zhigang Wu, Zhongxi Ding
date: 2026-03-21
publication date: 2026-03-21
starting page: 1396
ending page: 1403
doi: 10.5937/jomb0-61476

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subject: Original paper
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creator: Handong Huang
rights: Sva prava zadržana (c) 2025 Handong Huang
date: 2025-10-03
publication date: 2025-10-03
doi: 10.5937/jomb0-56191

The Novel Serum Biomarkers for Real-Time Treatment Monitoring and Prognostication in Hepatocellular Carcinoma Patients
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description: Background: Alpha-fetoprotein (AFP) has limited utility for real-time monitoring of hepatocellular carcinoma (HCC) during locoregional and systemic therapies. We evaluated six novel serum biomarkers—circulating tumor DNA (ctDNA), des-gamma-carboxy prothrombin (DCP), Golgi protein 73 (GP73), osteopontin (OPN), heat shock protein 70 (HSP70), and neutrophil-to-lymphocyte ratio (NLR)—against AFP for post-treatment response assessment and prognostication following transarterial chemoembolization (TACE) and sorafenib. Methods: A prospective cohort of 148 patients with intermediate-stage HCC undergoing TACE (n=86) or sorafenib (n=62) was enrolled. Serum samples were collected at baseline, week 4, week 8, and at radiologic progression. Biomarker kinetics were correlated with mRECIST response and overall survival (OS). Area under the receiver operating characteristic curve (AUC) was calculated for residual disease detection and progression prediction. Results: For post-TACE residual disease detection, HSP70 showed the highest sensitivity (88%), followed by GP73 (84%). For sorafenib monitoring, DCP and OPN independently predicted progression (AUC 0.86 and 0.83 respectively). A composite panel (DCP + GP73 + OPN + HSP70) achieved 92% specificity for early progression detection (AUC 0.93). Patients with persistent elevation of ≥3 markers at week 8 had significantly worse median OS (6.2 vs. 16.8 months, p<0.001). Conclusion: Novel serum biomarkers—particularly DCP for sorafenib monitoring, HSP70 and GP73 for post-TACE residual disease detection, and OPN for tumor burden tracking—enable effective real-time treatment monitoring and prognostication in HCC, outperforming AFP across multiple therapeutic contexts.
subject: Original paper
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creator: Jing Wang
creator: Qing Yang
creator: Lei Zhao
creator: Yiming Feng
rights: Sva prava zadržana (c) 2026 Jing Wang, Qing Yang, Lei Zhao, Yiming Feng
date: 2026-06-23
publication date: 2026-06-23
doi: 10.5937/jomb0-66749

Digitalni Biomarkeri i Medicinski Algoritmi u Srpskom Zdravstvenom Sistemu: Komparativna analiza znanja, stavova, prakse i ekonomske valuacije među ključnim zdravstvenim profesionalcima
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description: Pozadina: Digitalna transformacija zdravstvene zaštite koju karakterišu pojava digitalnih biomarkera i medicinskih algoritama, obećava redefinisanje zdravstvenog sistema. Međutim, uspešna implementacija zavisi od prihvatanja, spremnosti i ekonomske procene ključnih zainteresovanih strana kao što su biohemičari i kliničari. Ova studija pruža prvu analizu stavova, praksi, znanja i ekonomske procene u vezi sa digitalnom zdravstvenom dijagnostikom sa posebnim fokusom na dve podgrupe - biohemičare (medicinske/kliničke) i kliničare (patolozi/gastroenterolozi) kao predvodnicima digitalne implementacije. Metode: Sprovedeno je opsežno istraživanje sa kohortom od 105 zdravstvena radnika iz 53 istaknutih zdravstvenih ustanova. Studija je koristila kreirani strukturirani instrument za procenu digitalnih kompetencija, percipiranih koristi i potencijalnih rizika. Metoda kontingentne procene je korišćena za utvrđivanje spremnosti za plaćanje digitalnih medicinskih rešenja. Rezultati: Kohorta je pokazala visok nivo digitalne pismenosti (4.6 od mogućih 5). Biohemičari, čiji je rad utemeljen na analitičkoj validaciji, izrazili su veći oprez u pogledu kliničke relevantnosti i privatnosti podataka. Nasuprot tome, kliničari, vođeni potrebom za podrškom u dijagnostičkim odlukama, pokazali su oprez prema finansijskim temama. Analiza je izračunala optimalnu cenu od 65.9 ± 75.56 evra (95% interval poverenja: 41.2 – 90.6 evra). Nema značajne razlike između podgrupa (p = 0,89). Dok više od 75% učesnika veruje da će digitalni biomarkeri doneti sistemske finansijske uštede, 74% učesnika nije upoznato sa postojećim regulatornim okvirima. Zaključci: Srpska zdravstvena zajednica poseduje snažnu digitalnu osnovu. Biohemičari digitalna medicinska rešenja vide kao analitičke alate koji zahtevaju validaciju, dok ih kliničari vide kao rešenja za dijagnostičku dvosmislenost. Osnivanje nacionalne radne grupe za „Digitalnu dijagnostiku“ radi razvoja njihovih smernica i razvoja šifri za nadoknadu tro…
subject: Original paper
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creator: Dragisa Obradovic
creator: Đorđe Kralj
creator: Andrijana Milošević Georgiev
creator: Dragana Lakić
rights: Sva prava zadržana (c) 2026 Dragiša Obradović, Đorđe Kralj, Andrijana Milošević Georgiev, Dragana Lakić
date: 2026-06-23
publication date: 2026-06-23
doi: 10.5937/jomb0-64010

Diagnostic Value of Integrated Serum Protein Carbonyl, 8-Hydroxy-2'-Deoxyguanosine, and PGC-1α Profiling for Myocardial Energy Metabolism Disorder in Ischemic Cardiomyopathy
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description: Background: Patients with ischemic cardiomyopathy (ICM) often have abnormalities in myocardial energy metabolism, whereas conventional cardiac function indices may not capture metabolic injury in a timely manner. This study aimed to evaluate the diagnostic value of combined serum protein carbonyl (PC), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) detection for myocardial energy metabolism disorder in patients with ICM. Methods: A total of 167 patients with ICM hospitalized between June 2024 and October 2025 were retrospectively enrolled. According to myocardial energy metabolism status, they were divided into an energy metabolism disorder group (n=78) and a non-disorder group (n=89). Serum PC, 8-OHdG, and PGC-1α levels were measured. Clinical data, cardiac function indices, and serum biomarker levels were compared between the two groups. A combined diagnostic model was then constructed using logistic regression, and receiver operating characteristic (ROC) curves were used to evaluate the diagnostic performance of single indicators and combined detection. Results: Compared with the non-disorder group, the energy metabolism disorder group showed increased PC and 8-OHdG levels and decreased PGC-1α levels, with statistically significant differences (P<0.001). Multivariate logistic regression showed that PC, 8-OHdG, and PGC-1α were independently associated with myocardial energy metabolism disorder. ROC analysis showed that the areas under the curve (AUCs) of PC, 8-OHdG, and PGC-1α alone were 0.806, 0.791, and 0.772, respectively. The AUC of the combined diagnostic score increased to 0.884, with a sensitivity of 87.18% and a specificity of 79.78%, and the AUC of the combined model was higher than that of each single indicator. Conclusion: Increased serum PC and 8-OHdG levels and decreased PGC-1α levels are closely related to myocardial energy metabolism disorder in patients with ICM. Combined dete…
subject: Original paper
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creator: Qingli Wang
creator: Bei Liu
rights: Sva prava zadržana (c) 2026 Qingli Wang, Bei Liu
date: 2026-06-23
publication date: 2026-06-23
doi: 10.5937/jomb0-67769

Klinički i biohemijski prognostički značaj cirkulirajućih biomarkera povezanih sa tumorom kod pacijenata sa karcinomom pluća sa komorbidnom hroničnom opstruktivnom plućnom bolešću
type: item
description: Background: Chronic obstructive pulmonary disease (COPD) is closely associated with lung cancer development and progression through persistent systemic inflammation and immune-biochemical alterations. Circulating tumor-associated biochemical biomarkers may reflect disease severity and provide valuable prognostic information. This study aimed to explore the potential association between circulating tumor markers and prognosis in lung cancer patients with comorbid COPD, acknowledging the exploratory nature of this retrospective analysis. Methods: Clinicopathological characteristics and circulating biochemical biomarkers, including carcinoembryonic antigen (CEA), carbohydrate antigen 125 (CA125), carbohydrate antigen 153 (CA153), carbohydrate antigen 199 (CA199), cytokeratin-19 fragment (CYFRA21-1), and vascular endothelial growth factor (VEGF), were analyzed. Multivariate logistic regression was performed to identify independent prognostic biochemical predictors. Receiver operating characteristic (ROC) curve analysis was used to evaluate prognostic performance. Results: Patients with COPD demonstrated significantly elevated circulating levels of tumor-associated biochemical biomarkers compared with those without COPD (P<0.05). Multivariate analysis identified advanced TNM stage, lymph node metastasis, and increased serum concentrations of CYFRA21-1, CEA, CA125, CA153, CA199, and VEGF as independent biochemical predictors of poor prognosis (P<0.05). ROC curve analysis showed that CA199 (AUC>0.75), CA153 (AUC>0.75), and CA125 (AUC>0.75) exhibited strong prognostic performance, indicating their clinical utility for laboratory-based risk stratification. Conclusions: In this retrospective analysis, circulating tumor-associated biochemical biomarkers showed potential associations with prognosis in lung cancer patients with COPD. However, these findings are hypothesis-generating and require prospective validation before clinical implementation. The observed associations shou…
subject: Original paper
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creator: Hongxia Ma
creator: Qian Zhang
creator: Junnan Yue
creator: Jun Su
creator: Xingrui Meng
creator: Fangyuan Ren
creator: Zheng Li
rights: Sva prava zadržana (c) 2026 Hongxia Ma, Qian Zhang, Junnan Yue, Jun Su, Xingrui Meng, Fangyuan Ren, Zheng Li
date: 2026-04-13
publication date: 2026-04-13
starting page: 1232
ending page: 1242
doi: 10.5937/jomb0-65203

the Effect of Treatment on Serum C3, C4, IL-10, IL1-β, IL-6, TNF-α in Patients with Systemic Lupus Erythematosus
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description: -
subject: Original paper
  topic: section
subject: Array
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creator: Nadira Reyimu
creator: Yueyue Jing
rights: Sva prava zadržana (c) 2025 Nadira Reyimu, Yueyue Jing
date: 2025-12-03
publication date: 2025-12-03
starting page: 1383
ending page: 1395
doi: 10.5937/jomb0-62357

Анализа корелације нивоа сира СИР2Л4, Ц1КТНФ5 и ЦБП-35 у серуму са прогнозом пацијената са дијабетичком ретинопатијом
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description: Objective: To explore the relationships between serum Silent Mating Type Information Regulation 2 Homolog 4 (SIR2L4), C1q and tumor necrosis factor related protein 5 (C1QTNF5), Carbohydrate-binding protein 35 (CBP-35) and glycolipid metabolism and prognosis in patients with diabetic retinopathy (DR). Methods: The DR group was selected from among 230 hospitalised DR patients admitted between January 2023 and January 2024, including 122 non-proliferative DR patients (Non-proliferative DR group) and 108 proliferative DR patients (Proliferative DR group). Additionally, 100 subjects who underwent health check-ups in the hospital during the same period were selected as the control group. Indicators of SIR2L4, C1QTNF5, CBP-35, blood glucose [Fasting plasma glucose (FPG)], and the levels of blood lipids in the DR group and the control group were measured and compared. Triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and total cholesterol (TC) were among them. Moreover, the correlations between serum SIR2L4, C1QTNF5, and CBP-35 levels and blood glucose and lipid indicators in DR patients were analysed. The DR patients were monitored and followed up with for six months after treatment. Depending on their level of visual impairment, the patients were split into groups with excellent and poor prognoses. The levels of serum SIR2L4, C1QTNF5 and CBP-35 in the two groups were compared. Multivariate logistic regression was used to analyse the risk factors for poor prognosis in DR patients. A receiver operating characteristic (ROC) curve was used to analyse the predictive value of single or combined detection of SIR2L4, C1QTNF5, and CBP-35 for poor prognosis in patients with DR. Results: Pearson correlation analysis revealed that the levels of SIR2L4, C1QTNF5, and CBP-35 in DR patients were positively correlated with FPG, TG, TC, and LDL-C (P<0.05) and negatively correlated with HDL-C (P<0.05). The poor-prognosis group had…
subject: Original paper
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creator: Wenming Cheng
creator: Hua Lu
creator: Ting Song
creator: Wei Liu
rights: Sva prava zadržana (c) 2025 Wenming Cheng, Hua Lu, Ting Song, Wei Liu
date: 2025-11-13
publication date: 2025-11-13
starting page: 1373
ending page: 1382
doi: 10.5937/jomb0-61802

Дијагностичка вредност серума 25-хидроксивитамин Д, МИС, инсулин-сличан фактор раста 1 за синдром полицистичних јајника (ПЦОС)
type: item
description: Objective To investigate blood levels and the importance of Müllerian Inhibiting Substance (MIS), insulin-like growth factor 1 (IGF-1), insulin-like growth factor binding protein 3 (IGFBP-3), and 25-hydroxyvitamin D [25(OH)D] in patients with polycystic ovarian syndrome (PCOS). Methods The observation group consisted of 212 PCOS patients who received treatment at this institution between May 2022 and May 2024, while the control group consisted of 212 healthy women who were examined physically at same hospital within the same time frame. The two groups' general information, serum 25(OH)D, MIS, IGF-1, and IGFBP-3 levels, as well as their sex hormones and glucose metabolism markers, were compared. PCOS patients were divided into the IR group and the non-IR group according to whether insulin resistance (IR) occurred. The two groups' levels of serum 25(OH)D, MIS, IGF-1, and IGFBP-3 were compared. The study examined the associations among the four markers and the homeostasis model of insulin resistance index (HOMA-IR), as well as their supplementary diagnostic utility for PCOS. Results The level of 25(OH)D in the observation group was lower than that in the control group (P<0.05). Total testosterone (TT), luteinizing hormone (LH), estradiol (E2), fasting insulin (FINS), fasting blood glucose (FBG), serum MIS, IGF-1, IGFBP-3, HOMA-IR, and the average body mass index were all greater in the observation group than in the control group (P<0.05). The IR group's 25(OH)D level was lower than the non-IR group's (P<0.05). Serum MIS, IGF-1, and IGFBP-3 levels were higher in the IR group than in the non-IR group (P<0.05). The levels of serum MIS, IGF-1 and IGFBP-3 in the observation group were positively correlated with HOMA-IR (r=0.659, 0.586, 0.565, P<0.05), and 25(OH)D was negatively correlated with HOMA-IR (r=-0.548, P<0.05). The combined auxiliary diagnosis of PCOS by serum 25(OH)D, MIS, IGF-1, and IGFBP-3 had an area under the curve of 0.963, according to the results of receiv…
subject: Original paper
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subject: Array
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creator: Shaomeng Sun
creator: Jianrui Zheng
creator: Ziying Xie
rights: Sva prava zadržana (c) 2025 Shaomeng Sun, Jianrui Zheng, Ziying Xie
date: 2025-12-05
publication date: 2025-12-05
starting page: 1412
ending page: 1419
doi: 10.5937/jomb0-62488

Ефекти нивоа тромбоспондина-1 (THBS1) и тол-лика рецептора 4 (TLR-4) у серуму и синовијалној течности код пацијената са остеоартритисом колена
type: item
description: Objective To explore the changes in and significance of Thrombospondin-1 (THBS1) and Toll-like receptor 4 (TLR-4) levels in the serum and joint fluid of patients with knee osteoarthritis (KOA). Methods 184 KOA patients who were admitted to our hospital between May 2022 and February 2025 were chosen to serve as the observation group, and another 64 healthy adults who underwent physical examinations at the same hospital during the same period composed the control group. Tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, THBS1, and TLR-4 levels in the two groups' serum and joint fluid were compared. The correlations between the levels of TNF-α, IL-1β, THBS1 and TLR-4 in the serum and synovial fluid of the two groups and the severity of KOA were evaluated, and the clinical value of THBS1 and TLR-4 in the diagnosis of KOA was analyzed. Results TNF-α, IL-1β, THBS1, and TLR-4 levels in the observation group's serum and joint fluid were considerably higher than those in the control group (P<0.05). With an increase in Kellgren-Lawrence (K-L) grade, the observation group's serum and joint fluid had higher amounts of TNF-α, IL-1β, THBS1, and TLR-4. When TNF-α, IL-1β, THBS1, and TLR-4 levels were compared among K-L grades, statistically significant differences were found (P<0.05). The K-L grade of the patients was positively correlated with TNF-α, IL-1β, THBS1, and TLR-4 in the blood and joint fluid (P<0.05), and the factors of TNF-α, IL-1β, THBS1, and TLR-4 in the observation group's serum and joint fluid were positively correlated with each other (P<0.05). The sensitivity and specificity of serum THBS1 and TLR-4 for the diagnosis of KOA were 0.916 and 0.889 and 0.825 and 0.865, respectively, and the optimal cutoff values were 247.262 and 5.084 ng/mL, respectively. The sensitivity and specificity of THBS1 and TLR-4 in the joint fluid for diagnosing KOA were 0.945 and 0.932 and 0.985 and 0.916, respectively, and the optimal cutoff values were 239.745 and 4.620 ng/mL, respect…
subject: Original paper
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subject: Array
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creator: Yingjie Xu
creator: Zhen Tian
creator: Mingjin Liang
creator: Xiaoyun Li
rights: Sva prava zadržana (c) 2025 Yingjie Xu, Zhen Tian, Mingjin Liang, Xiaoyun Li
date: 2025-12-05
publication date: 2025-12-05
starting page: 1404
ending page: 1411
doi: 10.5937/jomb0-62485

Uticaj autoverifikacije na vreme izdavanja rezultata za kliničko-hemijske i imunoesejske testove
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description: Background: Auto-verification is increasingly recognized as a key tool for improving quality and efficiency in clinical laboratories. This study aimed to investigate the impact of an auto-verification system implemented for clinical chemistry and immunoassay tests on turnaround time (TAT) in a tertiary-care medical biochemistry laboratory. Methods: This study was conducted in the Medical Biochemistry Laboratory of XXX Hospital, a tertiary healthcare institution. Clinical chemistry and immunoassay tests were subjected to auto-verification using the navify® Lab Operations middleware (Roche Diagnostics, Germany) integrated with the MIA-MED Laboratory Information System (MIA Technology, Turkey). Algorithms were developed in accordance with CLSI AUTO10-A and AUTO15 guidelines, incorporating rules for quality control, serum indices, analyzer flags, delta checks, critical values, consistency checks, and analytical measurement intervals, as well as recommendations from the national health authorities. Validation of the algorithms was carried out using both simulated and patient data. The proportion of results exceeding predefined TAT targets was compared before and after auto-verification implementation with using the chi-square test. p<0.05 was considered statistically significant. Results Overall, 71% of test results and 21% of tube-based results were verified automatically. Median TAT was reduced by 6 minutes for emergency tests and 12 minutes for routine tests. The proportion of results exceeding the TAT threshold decreased significantly from 6.4% before auto-verification to 5.8% after auto-verification implementation (p < 0.001). Conclusions Auto-verification, with clearly defined and validated rules, enhances both the reliability and timeliness of laboratory results, thereby supporting quality improvement initiatives in clinical laboratories
subject: Original paper
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creator: Havva Yasemin Çinpolat
rights: Sva prava zadržana (c) 2026 Havva Yasemin Çinpolat
date: 2026-01-27
publication date: 2026-01-27
starting page: 1342
ending page: 1353
doi: 10.5937/jomb0-61380

PRIMENJIVOST FORMULA ZA IZRAČUAVANJE LDL-HOLESTEROLA U HIPERTRIGLICERIDEMIJI: UVIDI IZ VOJVODINE
type: item
description: Uvod: LDL-holesterol predstavlja ključni parametar u proceni rizika nastanka aterosklerotske kardiovaskularne bolesti. Određivanje LDL-holesterola direktnom metodom nije uvek moguće iz praktičnih ili finansijskih razloga, te izračunavanje putem formula ima značaj u njegovoj procenu. Cilj ovog istraživanja je bio da ispita primenljivost četiri različite formule za izračunavanje LDL-holesterola u odnosu na direktnu metodu kod pacijenata sa vrednostima serumskih triglicerida u između 4,5 do 9,0 mmol/L u populaciji Vojvodine. Metode: Ovo istraživanje je sprovedeno kao retrospektivna studija koja je uključila 272 ispitanika kod kojih su određivani parametri lipidskog statusa u periodu od juna 2022 do juna 2023. godine. Ispitanici su imali vrednosti triglicerida u serumu od 4,5 do 9,0 mmol/L. LDL-holesterol je određivan direktnom metodom na Alinity c analizatoru (Abbott), a vrednosti LDL-holesterola su i izračunavane formulama koje su predložili Friedewald, Sampson, Anandaraja i Martin. Rezultati: U ispitivanoj populaciji prosečna starost je bila 52 godine a medijana koncentracije triglicerida 5,48 mmol/L. Statistički značajna pozitivna korelacija postoji između direktno određivanog LDL-holesterola (d-LDL-C) i svih izračunatih vrednosti (p<0,001). Najmanja prosečna razlika je uočena između d-LDL-C i LDL-holesterola računatog po formuli Sampson i sar. (MD=-0,032). Poređenjem d-LDL-C i vrednosti LDL-holesterola dobijenih izračunavanjem, jedino formula Sampsona i sar. nije pokazala statističku značajnost razlika (p=0,240). Zaključak: U ispitivanoj populaciji pacijenata sa hipertrigliceridemijom, formula Sampsona i saradnika je pokazala najbolje performanse u odnosu na ostale ispitivane te bi njena primena mogla biti razmotrena kod stanovništva Vojvodine.
subject: Original paper
  topic: section
subject: Array
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creator: Dragana Žuvić
creator: Stanislava Nikolić
creator: Romana Mijović
creator: Branislava Ilinčić
creator: Aneta Ranđelović Živković
creator: Dušan Sedlarević
creator: Velibor Čabarkapa
rights: Sva prava zadržana (c) 2026 Dragana Žuvić, Stanislava Nikolić, Romana Mijović, Branislava Ilinčić, Aneta Ranđelović Živković, Dušan Sedlarević, Velibor Čabarkapa
date: 2026-02-04
publication date: 2026-02-04
starting page: 1354
ending page: 1363
doi: 10.5937/jomb0-61948

Анализа корелације нивоа експресије сировинског молекула за адхезију ендотелних ћелија-1 и протеина сиртуин 1 код акутног респираторног дистрес синдрома
type: item
description: Objective: To investigate the relationships among serum silencing information regulator 2-related enzyme 1 (SIRT1) expression levels, endothelial cell-specific molecule-1 (ESM-1), and fibroblast growth factor-21 (FGF21) and treatment results in patients with acute respiratory distress syndrome (ARDS) associated with sepsis. Methods: A total of 140 patients with sepsis-related ARDS were selected and divided into a good outcome group of 96 patients and a poor outcome group of 44 patients according to the treatment outcome. The levels of serum SIRT1, ESM-1 and FGF21 were compared between the two groups; the correlations between serum SIRT1, ESM-1, and FGF21 and the severity of the disease as well as the treatment outcome were analyzed; and the predictive value of serum SIRT1, ESM-1 and FGF21 for treatment outcomes was evaluated. Results: Compared to the group with a favorable outcome, the bad outcome group's serum SIRT1 level was lower, while the ESM-1 and FGF21 levels were significantly greater than those in the good outcome group (P<0.05). Serum SIRT1 levels steadily declined in patients with mild, moderate, and severe illness, while ESM-1 and FGF21 levels steadily rose (P<0.05). ESM-1 and FGF21 showed a positive association with illness severity (P<0.05), while serum SIRT1 showed a negative correlation (P<0.05) with disease severity, according to Spearman correlation analysis. Partial correlation analysis revealed that the serum SIRT1, ESM-1, and FGF21 levels were significantly correlated with the treatment outcomes of patients with sepsis-related ARDS (P<0.05). Serum SIRT1, ESM-1, and FGF21 levels and treatment outcomes were strongly correlated (P<0.05) in patients with sepsis-related ARDS. Serum SIRT1, ESM-1, and FGF21 areas under the curve (AUCs) for predicting the course of treatment for patients with ARDS associated with sepsis were 0.742, 0.838, and 0.796, respectively. The sensitivity was 77.27%, 77.27%, and 70.45%, and the specificity was 64.58%, 81.25%, and…
subject: Original paper
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subject: Array
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creator: Can Peng
creator: Zhigang Sun
creator: Yanyan Liu
creator: Hao Zhao
rights: Sva prava zadržana (c) 2025 Can Peng, Zhigang Sun, Yanyan Liu, Hao Zhao
date: 2025-11-29
publication date: 2025-11-29
starting page: 1364
ending page: 1372
doi: 10.5937/jomb0-61088

Clinical significance of miR-3178 in non-small cell lung cancer patients
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description: Background & objectives：Non-small cell lung cancer (NSCLC) is a malignant tumor with high morbidity and mortality, with low survival rate and poor prognosis This study aims to explore the diagnostic and prognostic value of miR-3178 in NSCLC. Methods: Study included 118 NSCLC patients and 97 healthy subjects. RT-qPCR measured miR-3178 and MIB2 in serum or cell lines. χ2 tests linked miR-3178 to clinicopathological features. ROC, Kaplan-Meier, and Cox analyses evaluated diagnostic and prognostic significance of miR-3178 and MIB2. Results: Compared to the control group, the expression level of miR-3178 was significantly reduced, while MIB2 expression was notably elevated in NSCLC patients. The expression of miR-3178 is significantly correlated with the TNM stage and LNM in NSCLC patients. NSCLC patients with higher miR-3178 expression levels exhibit significantly higher 5-year overall survival rates. Furthermore, TNM stage, miR-3178 expression, and MIB2 expression are independent risk factors that influence the prognosis of NSCLC. MIB2 serves as a target gene of miR-3178, and their expression levels display a significant negative correlation. When diagnosing NSCLC solely based on miR-3178, the AUC is 0.838; for MIB2 alone, the AUC is 0.818; however, when miR-3178 and MIB2 are combined for diagnosis, the AUC increases to 0.903. Conclusion: The downregulation of miR-3178 and upregulation of MIB2 are associated with the progression and deterioration of NSCLC patients, indicating poor prognosis. miR-3178 targets MIB2, and their combined diagnosis can enhance the diagnostic value for NSCLC, potentially reducing the risk of adverse outcomes.
subject: Original paper
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creator: Haizhen Yu
creator: Shizhen Li
rights: Sva prava zadržana (c) 2026 Haizhen Yu, Shizhen Li
date: 2026-01-23
publication date: 2026-01-23
starting page: 1332
ending page: 1341
doi: 10.5937/jomb0-57060

Serum OKS40 Ligand kao biohemijski regulator Th2-dominantne imune aktivacije u astmi: integrisana analiza citokinske mreže
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description: Background: Asthma is characterized by complex immune dysregulation involving coordinated cytokine and chemokine networks. OX40 ligand (OX40L), a key T-cell co-stimulatory molecule, has been implicated in Th2-mediated inflammation; however, its serum-level biochemical interactions with immune activation pathways in asthma remain incompletely defined. Methods: In this cross-sectional study, serum OX40L and immune activation-related factors were quantified in 82 patients with asthma and 40 healthy controls. Multiplex cytokine analysis was performed to measure Th2-, Th17-, pro- and anti-inflammatory mediators, along with chemokines and total IgE. Lung function indices were assessed by spirometry. Correlation analysis and multivariable linear regression were applied to identify immune factors independently associated with serum OX40L levels. Results: Serum OX40L concentrations were significantly elevated in asthma patients compared with controls and increased progressively with disease severity (all P<0.001). OX40L exhibited strong positive correlations with Th2-associated cytokines, particularly interleukin-5 (IL-5; r=0.75, P<0.001), and chemokines, while showing a significant inverse association with the anti-inflammatory cytokine IL-10. OX40L levels were also negatively correlated with forced expiratory volume in one second (FEV1%pred; r=-0.72, P<0.001). Multivariable regression identified IL-5 (β=0.35), FEV1%pred (β=-0.28), and annual exacerbation frequency (β=0.22) as independent biochemical determinants of serum OX40L, collectively explaining 68.3% of its variance. Conclusion: Serum OX40L is closely integrated within the Th2-dominant immune activation network in asthma and reflects both immune imbalance and functional airway impairment. These findings support OX40L as a biochemically relevant indicator of immune activation intensity and highlight its potential role within cytokine-driven regulatory pathways in asthma.
subject: Original paper
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creator: Ziyi Zhu
creator: Shumin Li
rights: Sva prava zadržana (c) 2026 Ziyi Zhu, Shumin Li
date: 2026-03-15
publication date: 2026-03-15
starting page: 1295
ending page: 1306
doi: 10.5937/jomb0-64381

Procena nivoa alfa klotoa u plazmi kod gojaznih slučajeva
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description: Pozadina: Kloto je gen protiv starenja koji produžava životni vek kada je prekomerno izražen i ubrzava starenje kada je poremećen. U našoj studiji ćemo istražiti korelacije između nivoa proteina a-Kloto (a-KL) u plazmi sa indeksom telesne mase (BMI), debljinom karotidne intime medije (CIMT), ukupnim antioksidativnim statusom (TAS), ukupnim oksidativnim statusom (TOS), indeksom oksidativnog stresa (OSI), visokosenzitivnim C-reaktivnim proteinom (hsCRP) i vrednostima holesterola kod gojaznih pacijenata. Metode: Ova studija je sprovedena na jednocentričan i poprečni način. Rezultati: Studija je sprovedena sa ukupno 90 učesnika, 35 žena (38,9%) i 55 muškaraca (61,1%). Od učesnika uključenih u studiju, 30 je imalo normalnu težinu (33,3%) (Grupa 1), 30 je imalo prekomernu težinu (33,3%) (Grupa 2) i 30 je bilo gojazno (33,3%) (Grupa 3). Postojale su statistički značajne razlike u vrednostima a-KL proteina (p=0,010), hsCRP (p<0,001), proceni insulinske rezistencije pomoću modela homeostaze (HOMA-IR) (p=0,022), vrednostima ispitivanih grupa. Nema značajne razlike između ispitivanih grupa u pogledu vrednosti CIMT (p=0,380), TOS (p=0,613), TAS (p=0,056) i OSI (p=0,943). Zaključci: U zaključku, u ovoj studiji, grupa sa prekomernom težinom imala je vrednosti a-KL proteina koje su bile značajno više od gojazne grupe, dok su vrednosti hsCRP bile značajno više u gojaznoj grupi u poređenju sa grupama sa prekomernom i normalnom težinom. Nije bilo značajnih razlika za vrednosti CIMT, TAS, TOS i OSI u ispitivanim grupama. Nije bilo značajnih korelacija između vrednosti a-KL proteina sa vrednostima BMI, hsCRP, CIMT, TAS, TOS i OSI učesnika u studiji.
subject: Original paper
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creator: ibrahim solak
creator: Ibrahim Guney
creator: Sekmenli
creator: Kurku
creator: Mehmet Ali Eryilmaz
rights: Sva prava zadržana (c) 2026 Ibrahim Solak, Ibrahim Guney, Nevin Sekmenli, Huseyin Kurku, Mehmet Ali Eryilmaz
date: 2026-01-23
publication date: 2026-01-23
starting page: 1307
ending page: 1315
doi: 10.5937/jomb0-60664

Profili biohemijskih i inflamatornih biomarkera u serumu u teškoj preeklampsiji i njihova klinička prediktivna vrednost
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description: Background: Preeclampsia is a pregnancy-specific hypertensive disorder characterized by systemic endothelial dysfunction, metabolic disturbances, and inflammatory activation. However, comprehensive biochemical and inflammatory biomarker profiles associated with disease severity, particularly severe preeclampsia, remain insufficiently defined. This study aimed to investigate serum biochemical and inflammatory markers in preeclampsia and to evaluate their clinical predictive value for severe disease. Methods: In this retrospective case–control study, pregnant women were classified into severe preeclampsia (n=30), mild preeclampsia (n=30), and normal pregnancy control groups (n=30). Serum biochemical parameters, including alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase (LDH), uric acid, creatinine, albumin, total bilirubin, triglycerides, and total cholesterol, were measured. Inflammatory markers, including C-reactive protein (CRP), interleukin-6 (IL-6), interleukin-8, tumor necrosis factor-α, procalcitonin, neutrophil-to-lymphocyte ratio, and platelet-to-lymphocyte ratio, were analyzed. Group comparisons, Spearman correlation analyses, multivariable logistic regression, and receiver operating characteristic (ROC) curve analyses were performed. Results: Both biochemical and inflammatory markers showed significant stepwise alterations across the three groups, with the most pronounced abnormalities observed in severe preeclampsia (P < 0.05). LDH, uric acid, CRP, IL-6, and albumin were significantly associated with disease severity. Multivariable logistic regression identified elevated LDH, uric acid, CRP, IL-6, and reduced albumin as independent predictors of severe preeclampsia. ROC analysis demonstrated that the combined biomarker model achieved good discriminative performance, with an area under the curve of 0.89 (95% CI: 0.81–0.96). Conclusions: Severe preeclampsia is characterized by distinct serum biochemical and inflammatory biomarker p…
subject: Original paper
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subject: Array
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creator: Fan Yang
creator: Yanping Liu
creator: Yanyan Liu
rights: Sva prava zadržana (c) 2026 Fan Yang, Yanping Liu, Yanyan Liu
date: 2026-03-03
publication date: 2026-03-03
starting page: 1316
ending page: 1322
doi: 10.5937/jomb0-63709

Анализа корелације серумског ФГЛП1, УАЦР и ризика од срчане инсуфицијенције код пацијената са дијабетесом типа 2
type: item
description: Objective To explore the correlations between serum FGLP1 (Fibrinogen-like protein 1 C-terminal peptide) levels, the urine albumin-to-creatinine ratio (UACR) and left ventricular diastolic dysfunction (LVDD) in elderly patients with type 2 diabetes mellitus (T2DM). Methods From August 2023 to August 2025, 256 elderly patients with T2DM who were admitted to our hospital were selected as the case group, and 120 healthy individuals who underwent physical examinations were selected as the control group. In accordance with the diagnostic criteria for LVDD, T2DM patients were divided into a disorder group (n=70) and a nondisorder group (n=186), and single-factor analysis, multivariate logistic regression analysis, and Spearman correlation analysis were performed to explore the correlation between serum FGLP1 levels, UACRs and LVDD in T2DM patients, and receiver operating characteristic curves were used to evaluate the ability of serum FGLP1 levels and urine UACR to predict LVDD. Results The serum FGLP1 concentration and urine UACR were higher in the case group than in the healthy group (P<0.05). There were statistically significant differences in age, disease duration, serum FGLP1 concentration and UACR between the LVDD group and the non-LVDD group (P<0.05). Multivariate logistic regression analysis revealed that age ≥ 75 years, long disease duration, elevated UACR, and elevated serum FGLP1 concentration were independent risk factors for LVDD in patients with T2DM (P<0.05). Spearman correlation analysis indicated a positive association between LVDD and serum FGLP1 levels as well as UACR (r = 0.551, 0.559; both P<0.05). The areas under the curve for predicting LVDD by serum FGLP1 concentration, urine UACR and the prediction model were 0.848, 0.896 and 0.956, respectively. Conclusion Serum FGLP1 levels and urinary UACR in elderly patients with type 2 diabetes mellitus are closely related to LVDD. The combined detection of these two indicators is helpful for the early diagno…
subject: Original paper
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creator: Lianheng Xia
creator: Yingshuo Guo
creator: Jiaxin Wang
creator: Tingxuan Wang
creator: Chunhui Xu
creator: Meiyu Song
creator: Chang Du
creator: Qingjie Wang
creator: Jie Gao
rights: Sva prava zadržana (c) 2026 Lianheng Xia, Yingshuo Guo, Jiaxin Wang, Tingxuan Wang, Chunhui Xu, Meiyu Song, Chang Du, Qingjie Wang, Jie Gao
date: 2026-04-14
publication date: 2026-04-14
starting page: 1323
ending page: 1331
doi: 10.5937/jomb0-65295

Анализа корелације серума CCL3, TRACP-5b и склеростина са прогнозом пацијената са мултиплим миеломом
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description: [Objective] To investigate the clinical use of Sclerostin, tartrate-resistant acid phosphatase 5b (TRACP-5b), and serum C-C motif chemokine ligand 3 (CCL3) levels in assessing the health and prognosis of patients with multiple myeloma. [Methods] 250 patients with multiple myeloma who visited the hospital between January 2023 and December 2024 were selected as the observation group, whereas 150 healthy individuals who were evaluated there during that time made up the control group. The levels of serum CCL3, TRACP-5b and Sclerostin in the observation group before and after treatment were compared, as were the levels of serum CCL3, TRACP-5b and Sclerostin. The levels of serum CCL3, TRACP-5b and Sclerostin in MM patients with different tumor stages and different degrees of bone destruction were compared, and the univariate and multivariate factors of death within one year in MM patients were analyzed. To compare the predictive efficacy of single and combined detection of serum CCL3, TRACP-5b and Sclerostin for death within one year in patients with multiple myeloma. [Results] The levels of serum CCL3, TRACP-5b and Sclerostin in the observation group before and after treatment were significantly greater than those in the control group, and the differences were statistically significant (P<0.05). After treatment, the levels of serum CCL3, TRACP-5b and Sclerostin in the observation group were significantly lower than those before treatment, and the differences were statistically significant (P<0.05). There were statistically significant differences in tumor stage, classification of degree of bone destruction, CCL3 level, TRACP-5b level and Sclerostin level (P<0.05). Multivariate analysis revealed that tumor stage, the degree of bone destruction, the CCL3level, the TRACP-5b level and the Sclerostin level were the factors influencing death within one year in patients with multiple myeloma (P<0.05). The levels of serum CCL3, TRACP-5b and Sclerostin have relatively high predic…
subject: Original paper
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creator: Cuimei Jiang
creator: ManMan Sun
creator: Dashan Gao
creator: Xiaoli Zhang
creator: Jianan Pan
rights: Sva prava zadržana (c) 2025 Cuimei Jiang, ManMan Sun, Dashan Gao, Xiaoli Zhang, Jianan Pan
date: 2025-12-21
publication date: 2025-12-21
starting page: 1286
ending page: 1294
doi: 10.5937/jomb0-62533

Predviđanje promene morfologije n. vagus-a na osnovu biohemijski markera upale u COVID 19 infekciji
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description: Сажетак Циљ. Предвиђање површине попречног пресека нерва вагус-а на сонограму, на основу вредности неких биомаркера запаљења у крви. Методе. Код 68 пацијената са ПЦР(+) тестом за SARS-CoV 2, ултразвуком је мерена површина попречног пресека нерва (CSA), просeк од три мерења. Одређене су вредности CRP, фибриногена и феритина. Вредности CSA са обе стране и сви биомаркери су класификовани као (0)- у границама нормале и (1)- све остале вредности. Урађена је логистичка линеарна регресија и искључена је јака линеарна веза између предиктора. Формирана је неуронска мрежа и модел бинарне логистичке регресије за вредности CSA на десној страни, на левој страни није пронађена веза између одабраних предиктора и CSA вагуса. Резултати. На десној страни, ЦСА је 1,32±0,3; на левој страни 1,2±0,28. У студији, 11,8%, 22,1% и 8,82% имало је нормалне вредности CRP, фибриногена и феритина. Снажна линеарна повезаност предиктора искључена је линеарном логистичком регресијом. Неуронска мрежа са два скривена слоја и 6 јединица у сваком скривеном слоју формирана је за 2 секунде. Проценат тачности модела у обуци је 91,5%, а на тестирању је 90,5%. AUC овог модела је 0,76 (p=0,018). Логистички бинарни регресиони модел: logitX2D = 18,018+ (20,752к вредности CRP)+ (-38,77к вредности фибриногена)+(2,175к вредности феритина), где је X2D- површина попречног пресека десног н. вагусa, датa моделом. Exp(Б) = 7,5, Psample = 0,88, ОR=56,25, тачност модела је 91,2%, Омнибус тест: χ2=14,193, п=0,003. Коефицијент поузданости модела -2LodLH=35,068 и AUC =0,76, p=0,018. Укупан квалитет модела 0,61. На левој страни, вредност CSA н.вагус-a се не може предвидети коришћењем изабраних предиктора.
subject: Original paper
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creator: Olivera Jovanikic
rights: Sva prava zadržana (c) 2026 Olivera Jovanikic
date: 2026-03-31
publication date: 2026-03-31
starting page: 1251
ending page: 1258
doi: 10.5937/jomb0-57970

Dijagnostika hronične bubrežne bolesti u Srbiji: aktuelna laboratorijska praksa i prioriteti harmonizacije
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description: Sažetak Uvod Cilj ovog istraživanja bio je da se proceni aktuelna laboratorijska praksa u dijagnostici hronične bolesti bubrega (HBB) u Republici Srbiji i identifikuju oblasti koje zahtevaju harmonizaciju u skladu sa nacionalnim i međunarodnim preporukama. Metode Sprovedeno je presečno istraživanje zasnovano na upitniku u periodu jun–avgust 2025. godine, uz podršku Društvamedicinskih biohemičara Srbije, koje je distribuiralo pozive svojim članovima zaposlenim u medicinsko-biohemijskim laboratorijama širom Srbije. U istraživanju su učestvovale 83 laboratorije (stopa odgovora 84,7%), koje su obuhvatile primarni, sekundarni, tercijarni i privatni sektor zdravstvene zaštite. Podaci su analizirani deskriptivno. Rezultati Sve laboratorije određuju koncentraciju kreatinina u serumu, ali ostale ključne komponente dijagnostike HBB nisu dosledno implementirane. Procena jačine glomerulske filtracije (eGFR) zasnovana na kreatininu izračunava se u 63,9% laboratorija, dok se automatski prikazuje uz svaki rezultat kreatinina u 34,9%. Testiranje albuminurije dostupno je u 55,4% laboratorija; kvantitativno određivanje albumina sprovodi se u 31,3%, dok se odnos albumin/kreatinin (ACR) izračunava u 7,2% laboratorija. Određivanje cistatina C u serumu dostupno je u 7,2% laboratorija. U manje od polovine laboratorija (43,4%) istovremeno je dostupno i izračunavanje eGFR i testiranje albuminurije. Uočene su razlike u analitičkim metodama, referentnim intervalima, tipu uzorka urina i modelima izveštavanja. Integrisana primena eGFR i ACR najčešća je u privatnom sektoru, a najređa na nivou primarne zdravstvene zaštite. Zaključak Laboratorijska dijagnostika HBB u Srbiji i dalje je dominantno zasnovana na kreatininu, uz ograničeno određivanjealbuminurije i automatsko izveštavanja eGFR. Šira implementacija ACR i standardizacija izveštavanja neophodne su radi ranijeg otkrivanja i adekvatne procene rizika kod bolesnika sa HBB.
subject: Original paper
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creator: Vera Lukić
creator: Dušica Mrdaković
creator: Neda Milinković
rights: Sva prava zadržana (c) 2026 Vera Lukić, Dušica Mrdaković, Neda Milinković
date: 2026-03-26
publication date: 2026-03-26
starting page: 1276
ending page: 1285
doi: 10.5937/jomb0-65067